Coding

Part:BBa_K4868998:Design

Designed by: Maria Bjerregaard   Group: iGEM23_SDU-Denmark   (2023-10-12)


The coding sequence of the dehalogenase 4 gene from Delftia acidovorans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 531
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 238
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PaqCI restriction sites were made to flank the coding sequence to allow for easy Golden Gate cloning. The coding sequence was codon-optimized for E. coli.



Source

BBa_K3723006

References